1F9F

CRYSTAL STRUCTURE OF THE HPV-18 E2 DNA-BINDING DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Detector technology	IMAGE PLATE
Collection date	1999-08-14
Detector	RIGAKU RAXIS
Spacegroup name	P 21 21 21
Unit cell lengths	41.500, 46.800, 161.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.520 - 1.900
R-factor	0.237
Rwork	0.237
R-free	0.29400
RMSD bond length	0.028
RMSD bond angle	23.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000 *
High resolution limit [Å]	1.900 *	1.900 *
Rmerge	0.078 *
Number of reflections	25003 *
Completeness [%]	97.5 *	88.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	Tris-HCl	25 (mM)
3	1	drop		100 (mM)
4	1	drop	dithiothreitol	10 (mM)
5	1	reservoir	ammonium sulfate	2.7 (M)
6	1	reservoir	sodium acetate	0.1 (M)