1F9B

MELANIN PROTEIN INTERACTION: X-RAY STRUCTURE OF THE COMPLEX OF MARE LACTOFERRIN WITH MELANIN MONOMERS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	283
Detector technology	IMAGE PLATE
Collection date	1998-05-07
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	85.037, 99.815, 103.425
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.700
R-factor	0.215
Rwork	0.215
R-free	0.28700
RMSD bond length	0.014 *
RMSD bond angle	2.000 *
Data reduction software	DENZO
Phasing software	AMoRE
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	15.000
High resolution limit [Å]	2.700	2.700
Rmerge	0.071 *	0.142 *
Total number of observations	100340 *
Number of reflections	18557
<I/σ(I)>	3.6
Completeness [%]	91.0	63 *
Redundancy	2.3	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	5	277	20mM Tris-Hcl, pH 5.0 concentration 50 mg/ml 10% ethanol soaked for 12hours in buffer containing DOPA, MICRODIALYSIS, temperature 4K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Tris-HCl	20 (mM)
2	1	1	protein	50 (mg/ml)
3	1	2	ethanol	10 (%)