1F97

SOLUBLE PART OF THE JUNCTION ADHESION MOLECULE FROM MOUSE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-21
Temperature [K]	120
Detector technology	AREA DETECTOR
Collection date	1998-08-06
Detector	SIEMENS HI-STAR
Spacegroup name	I 2 2 2
Unit cell lengths	42.540, 83.280, 130.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.500
R-factor	0.15
Rwork	0.150
R-free	0.21000
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHARP
Refinement software	X-PLOR (98.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.054	0.130 *
Total number of observations	18966 *
Number of reflections	7497 *
<I/σ(I)>	14.4
Completeness [%]	89.5	68.3
Redundancy	2.5	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.5	293	25% Peg 3350, 200 mM MgCl2, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-15 (mg/ml)
2	1	reservoir	PEG3350	25 (%)
3	1	reservoir		200 (mM)
4	1	reservoir	Tris	100 (mM)