1F91

BETA-KETOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE I IN COMPLEX WITH C10 FATTY ACID SUBSTRATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	263
Detector technology	IMAGE PLATE
Collection date	1998-06-10
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	59.500, 141.500, 213.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	500.000 - 2.400
Rwork	0.174
R-free	0.21000 *
RMSD bond length	0.007 *
RMSD bond angle	1.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	500.000
High resolution limit [Å]	2.400
Rmerge	0.052 *
Number of reflections	70763
Completeness [%]	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4	294	1.9 M ammonium sulfate, 2% PEG400, 0.1 M bis-Tris propane pH 6.5, pH 7.4, VAPOR DIFFUSION, temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	reservoir	ammonium sulfate	1.9 (M)
3	1	reservoir	PEG400	2 (%)
4	1	reservoir	bis-Tris propane	0.1 (M)