1F90

FAB FRAGMENT OF MONOCLONAL ANTIBODY (LNKB-2) AGAINST HUMAN INTERLEUKIN-2 IN COMPLEX WITH ANTIGENIC PEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	300
Detector technology	IMAGE PLATE
Collection date	1999-02-05
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 1 21 1
Unit cell lengths	46.830, 72.760, 72.150
Unit cell angles	90.00, 106.98, 90.00

Refinement procedure

Resolution	99.000 - 2.600
R-factor	0.18 *
Rwork	0.165
R-free	0.26300 *
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.730
High resolution limit [Å]	2.600	2.600
Rmerge	0.112 *	0.583
Total number of observations	19271 *
Number of reflections	9377 *
<I/σ(I)>	6.9
Completeness [%]	99.7 *	97.15
Redundancy	4.3	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	300	PEG-4000, 2-propanol, Na-citrate , pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-7 (mg/ml)
2	1	reservoir	PEG4000	12-13 (%)
3	1	reservoir	2-propanol	12-13 (%)
4	1	reservoir	sodium citrate	60 (mM)