1F8W
CRYSTAL STRUCTURE OF NADH PEROXIDASE MUTANT: R303M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-08-08 |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 155.180, 155.180, 189.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.450 |
| R-factor | 0.196 * |
| Rwork | 0.196 |
| R-free | 0.23400 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 3.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.048 * | 0.363 |
| Total number of observations | 60231 * | |
| Number of reflections | 36615 | |
| <I/σ(I)> | 9.5 | 2.6 |
| Completeness [%] | 85.8 | 70.9 * |
| Redundancy | 5.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.2 | 296 | 2% PEG 400, 2.0 M (NH4)2SO4, 100 mM Na Hepes, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG400 | 2-8 (%) | |
| 2 | 1 | drop | ammonium sulfate | 1.6-1.9 (M) | |
| 3 | 1 | drop | sodium HEPES | 0.1 (M) | |
| 4 | 1 | drop | protein | 7.5-10 (mg/ml) |
