1F8V

THE STRUCTURE OF PARIACOTO VIRUS REVEALS A DODECAHEDRAL CAGE OF DUPLEX RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	90
Detector technology	CCD
Collection date	1999-11-14
Detector	OTHER
Spacegroup name	P 1 21 1
Unit cell lengths	329.332, 346.944, 424.893
Unit cell angles	90.00, 90.83, 90.00

Refinement procedure

Resolution	20.000 - 3.000
R-factor	0.218
Rwork	0.219
R-free	0.22100
Structure solution method	molecular replacement and electron density averaging
Starting model (for MR)	crystal structure of flock house virus
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	GLRF
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	207.000	3.050
High resolution limit [Å]	3.000	3.000
Rmerge	0.105	0.266
Total number of observations	3226875 *
Number of reflections	1505825	76058 *
<I/σ(I)>	8.9	3.3
Completeness [%]	81.2	82.2
Redundancy	2.14	1.79

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEG 8000, lithium sulphate, calcium chloride, Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 20K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEG 8000, lithium sulphate, calcium chloride, Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 20K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 8000
2	1	1	Li2SO4
3	1	1	CaCl2
4	1	1	Tris-HCl

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		75 (mM)
2	1	reservoir		5 (mM)
3	1	reservoir	PEG8000	4 (%(w/v))
4	1	reservoir		50 (mM)
5	1	drop	protein	20 (mg/ml)
6	1	drop	Tris-HCl	50 (mM)