1F8V
THE STRUCTURE OF PARIACOTO VIRUS REVEALS A DODECAHEDRAL CAGE OF DUPLEX RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 1999-11-14 |
Detector | OTHER |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 329.332, 346.944, 424.893 |
Unit cell angles | 90.00, 90.83, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.218 |
Rwork | 0.219 |
R-free | 0.22100 |
Structure solution method | molecular replacement and electron density averaging |
Starting model (for MR) | crystal structure of flock house virus |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | GLRF |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 207.000 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.105 | 0.266 |
Total number of observations | 3226875 * | |
Number of reflections | 1505825 | 76058 * |
<I/σ(I)> | 8.9 | 3.3 |
Completeness [%] | 81.2 | 82.2 |
Redundancy | 2.14 | 1.79 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 8000, lithium sulphate, calcium chloride, Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 20K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 8000, lithium sulphate, calcium chloride, Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 20K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 8000 | ||
2 | 1 | 1 | Li2SO4 | ||
3 | 1 | 1 | CaCl2 | ||
4 | 1 | 1 | Tris-HCl |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 75 (mM) | ||
2 | 1 | reservoir | 5 (mM) | ||
3 | 1 | reservoir | PEG8000 | 4 (%(w/v)) | |
4 | 1 | reservoir | 50 (mM) | ||
5 | 1 | drop | protein | 20 (mg/ml) | |
6 | 1 | drop | Tris-HCl | 50 (mM) |