1F8E

Native Influenza Neuraminidase in Complex with 4,9-diamino-2-deoxy-2,3-dehydro-N-acetyl-neuraminic Acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	1996-09-22
Detector	RIGAKU RAXIS II
Spacegroup name	I 4 3 2
Unit cell lengths	181.130, 181.130, 181.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.400
Rwork	0.193
R-free	0.22100
RMSD bond length	0.011
RMSD bond angle	1.780
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	1.440
High resolution limit [Å]	1.400	1.400
Rmerge	0.080
Total number of observations	627381 *
Number of reflections	96246
<I/σ(I)>	10.3
Completeness [%]	97.7	99.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.9	293	Laver, W.G., (1984) Virology, 137, 314 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	potassium phosphate	1.7 (M)
2	1	drop	protein	10-15 (mg/ml)
3	1	reservoir	potassium phosphate	1.9 (M)