1F7Z
RAT TRYPSINOGEN K15A COMPLEXED WITH BOVINE PANCREATIC TRYPSIN INHIBITOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-06-24 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 92.696, 92.696, 62.076 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.550 |
| R-factor | 0.199 * |
| Rwork | 0.199 |
| R-free | 0.20900 |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 1.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.056 | 0.036 |
| Total number of observations | 172527 * | |
| Number of reflections | 44058 | |
| <I/σ(I)> | 31.5 | |
| Completeness [%] | 95.7 | 89.5 |
| Redundancy | 4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-15 (mg/ml) | |
| 2 | 1 | drop | 1 (mM) | ||
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | BPTI | ||
| 5 | 1 | reservoir | PEG3350 | 26 (%) | |
| 6 | 1 | reservoir | 0.2 (M) | ||
| 7 | 1 | reservoir | Tris | 0.1 (M) |
