1F7U
CRYSTAL STRUCTURE OF THE ARGINYL-TRNA SYNTHETASE COMPLEXED WITH THE TRNA(ARG) AND L-ARG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-06-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 129.646, 107.472, 71.379 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.980 - 2.200 |
| R-factor | 0.19 * |
| Rwork | 0.190 |
| R-free | 0.23300 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.089 | 0.210 |
| Number of reflections | 51493 | |
| <I/σ(I)> | 23.4 | |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 7 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | Delagoutte, B., (2000) Acta Crystallogr., Sect.D, 56, 492. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | hexanediol | ||
| 2 | 1 | 1 | (NH4)2SO4 | ||
| 3 | 1 | 2 | (NH4)2SO4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ArgRS | 5 (mg/ml) | |
| 2 | 1 | drop | L-Arg | 5 (mM) | |
| 3 | 1 | drop | tRNA | 2 (mg/ml) | |
| 4 | 1 | drop | 20 (mM) | ||
| 5 | 1 | drop | ATP | 10 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 7 | 1 | reservoir | 1,6-hexanediol | 5 (%) | |
| 8 | 1 | reservoir | sodium cacodylate | 100 (mM) |
