1F6L

VARIABLE LIGHT CHAIN DIMER OF ANTI-FERRITIN ANTIBODY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1998-10-19
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 61 2 2
Unit cell lengths	52.214, 52.214, 157.750
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	500.000 - 2.800
Rwork	0.191
R-free	0.25700
RMSD bond length	0.007 *
RMSD bond angle	1.340 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.099	0.323
Total number of observations	40650 *
Number of reflections	3589 *
Completeness [%]	96.3	90.5
Redundancy	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	4 *	PEG 400, ammonium sulfate, hepes, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	domain	9-10 (mg/ml)
2	1	reservoir	PEG400	4 (%)
3	1	reservoir	ammonium sulfate	2.2 (M)
4	1	reservoir	HEPES	0.1 (M)	pH7.2