1F5W

DIMERIC STRUCTURE OF THE COXSACKIE VIRUS AND ADENOVIRUS RECEPTOR D1 DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-02-18
Detector	ADSC
Spacegroup name	P 43 21 2
Unit cell lengths	68.337, 68.337, 146.356
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.16
Rwork	0.158
R-free	0.19300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kac chain B
RMSD bond length	0.016
RMSD bond angle	0.030
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.840
High resolution limit [Å]	1.700	1.700
Rmerge	0.079 *	0.302 *
Number of reflections	36389	3806 *
<I/σ(I)>	2.4	0.4
Completeness [%]	94.1	70.2
Redundancy	3.7	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2 *	20 *	ammonium sulphate, sodium citrate, glycerol, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium acetate	100 (mM)
2	1	reservoir	glycerol	25 (%(v/v))
3	1	reservoir	ammonium sulfate	45-60 (%(v/v)sat)
4	1	drop	protein	12 (mg/ml)