1F5S
CRYSTAL STRUCTURE OF PHOSPHOSERINE PHOSPHATASE FROM METHANOCOCCUS JANNASCHII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-04-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.91165 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 69.000, 69.965, 91.238 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.800 |
| R-factor | 0.198 * |
| Rwork | 0.198 |
| R-free | 0.23500 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.250 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.053 | 0.305 |
| Number of reflections | 71908 | 2219 * |
| <I/σ(I)> | 22.4 | |
| Completeness [%] | 95.4 | 78.2 |
| Redundancy | 3.3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | 22% PEG 200 MME, 0.2M sodium phosphate, 0.1M Acetate buffer, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 20 (mM) | |
| 2 | 1 | drop | 0.3 (M) | ||
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | dithiothreitol | 10 (mM) | |
| 5 | 1 | drop | protein | 54 (mg/ml) | |
| 6 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 7 | 1 | reservoir | sodium phosphate dihydrate | 0.5 (M) | |
| 8 | 1 | reservoir | PEG2000 MME | 22 (%) |
