1F5M
STRUCTURE OF THE GAF DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-01-23 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 73.640, 73.640, 162.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 500.000 - 1.900 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.24600 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.725 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.057 | 0.545 |
| Total number of observations | 901143 * | |
| Number of reflections | 36203 | |
| <I/σ(I)> | 27.9 | |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | ammonium sulfate, lithium sulfate, Tris buffer, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | |
| 3 | 1 | drop | dithiothreitol | 2 (mM) | |
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 5 | 1 | reservoir | 0.2 (M) | ||
| 6 | 1 | reservoir | ammonium sulfate | 2.0 (M) |
