1F5C
CRYSTAL STRUCTURE OF F25H FERREDOXIN 1 MUTANT FROM AZOTOBACTER VINELANDII AT 1.75 ANGSTROM RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 115 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-12-11 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 55.222, 55.222, 92.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.050 - 1.750 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.22700 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.072 | 0.606 |
| Number of reflections | 15118 | |
| <I/σ(I)> | 8.8 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 13.42 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | Trizma base, ammonium sulfate, potassium phosphate, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277.0K |
