1F59
IMPORTIN-BETA-FXFG NUCLEOPORIN COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-13 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 67.246, 211.790, 125.815 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.800 |
| Rwork | 0.225 |
| R-free | 0.26200 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.089 | 0.176 |
| Total number of observations | 144382 * | |
| Number of reflections | 31295 | |
| <I/σ(I)> | 6 | |
| Completeness [%] | 69.1 | 10.8 |
| Redundancy | 4.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | ammonium sulphate, ammonium acetate, dtt, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 20K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 1-442 | 2.1 (mg/ml) | 0.003ml |
| 2 | 1 | drop | FF5 | 11 (mg/ml) | 0.001ml |
| 3 | 1 | drop | ammonium sulfate | 1.28-1.33 (M) | |
| 4 | 1 | drop | ammonium acetate | 200 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.24 (M) | |
| 6 | 1 | reservoir | ammonium acetate | 100 (mM) | |
| 7 | 1 | reservoir | dithiothreitol | 50 (mM) |
