1F51
A TRANSIENT INTERACTION BETWEEN TWO PHOSPHORELAY PROTEINS TRAPPED IN A CRYSTAL LATTICE REVEALS THE MECHANISM OF MOLECULAR RECOGNITION AND PHOSPHOTRANSFER IN SINGAL TRANSDUCTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.974, 117.774, 170.736 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 3.000 |
| R-factor | 0.228 * |
| Rwork | 0.228 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1SRR AND 1IXM |
| RMSD bond length | 0.014 * |
| RMSD bond angle | 1.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 3.140 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.076 * | |
| Total number of observations | 122403 * | |
| Number of reflections | 29914 | |
| <I/σ(I)> | 18 | 3.1 |
| Completeness [%] | 99.0 | 98.7 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.1 | 0.5M KCL, 24% PEG2K AND ALF3 AT pH8.1 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Spo0F | 9 (mg/ml) | |
| 2 | 1 | drop | Spo0B | 10.3 (mg/ml) | |
| 3 | 1 | drop | 2 (mM) | ||
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | drop | 30 (mM) | ||
| 6 | 1 | reservoir | 0.5 (M) | ||
| 7 | 1 | reservoir | PEG2000 | 24 (%) | |
| 8 | 1 | reservoir | Tris-HCl | 100 (mM) |
