1F4Z
BACTERIORHODOPSIN-M PHOTOINTERMEDIATE STATE OF THE E204Q MUTANT AT 1.8 ANGSTROM RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-01-05 |
Detector | ADSC QUANTUM |
Spacegroup name | P 63 |
Unit cell lengths | 60.631, 60.631, 108.156 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 12.000 - 1.800 |
R-factor | 0.131 * |
Rwork | 0.142 |
R-free | 0.20300 |
Starting model (for MR) | ISOMORPHOUS WITH 1C3W |
RMSD bond length | 0.009 |
RMSD bond angle | 0.024 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.082 | 0.446 |
Total number of observations | 214773 * | |
Number of reflections | 21451 | |
<I/σ(I)> | 28.2 | 3.3 |
Completeness [%] | 99.5 | 95.1 |
Redundancy | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Cubic lipid phase | 5.6 | 20 * | Landau, E.M., (1996) Proc.Natl.Acad.Sci.USA., 93, 14532. * |
1 | Cubic lipid phase | 5.6 | 20 * | Landau, E.M., (1996) Proc.Natl.Acad.Sci.USA., 93, 14532. * |
1 | Cubic lipid phase | 5.6 | 20 * | Landau, E.M., (1996) Proc.Natl.Acad.Sci.USA., 93, 14532. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | lipids | 60-70 (%(w/v)) | |
2 | 1 | 1 | BR | 2.5-4.5 (mg/ml) | |
3 | 1 | 1 | sodium potassium phosphate | 0.7-4.0 (M) | |
4 | 1 | 1 | MPD | 1.5-3.75 (%) | |
5 | 1 | 1 | OG | 0.36-0.48 (%) |