1F4L

CRYSTAL STRUCTURE OF THE E.COLI METHIONYL-TRNA SYNTHETASE COMPLEXED WITH METHIONINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-08
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	78.400, 45.160, 86.310
Unit cell angles	90.00, 107.91, 90.00

Refinement procedure

Resolution	20.000 - 1.850
Rwork	0.182
R-free	0.23900
RMSD bond length	0.010
RMSD bond angle	0.028
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.850
Rmerge	0.060
Total number of observations	130975 *
Number of reflections	45164
<I/σ(I)>	15.4
Completeness [%]	91.2
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	micro-seeding *	7.3 *	20 *	ammonium citrate, methyl-pentanediol, L-methionine, mercaptoethanol, phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop		10 (mM)
3	1	drop	beta-mercaptoethanol	10 (mM)
4	1	reservoir	ammonium citrate	1.1 (M)
5	1	reservoir	MPD	0.5 (%(v/v))
6	1	reservoir	L-methionine	0.6 (mM)
7	1	reservoir	beta-mercaptoethanol	2 (mM)
8	1	reservoir	phosphate	30 (mM)