1F4K

CRYSTAL STRUCTURE OF THE REPLICATION TERMINATOR PROTEIN/B-SITE DNA COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	1999-12-16
Detector	ADSC QUANTUM 4
Spacegroup name	P 32 2 1
Unit cell lengths	44.780, 44.780, 395.580
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.232 *
Rwork	0.228
R-free	0.27590
RMSD bond length	0.010
RMSD bond angle	1.720
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.580
High resolution limit [Å]	2.500	2.500
Rmerge	0.073	0.175
Total number of observations	147110 *
Number of reflections	15166 *
<I/σ(I)>	13.7
Completeness [%]	87.9	54.2
Redundancy	9.7	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	21 *	5% PEG 4000, 0.1M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP at 298K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	sodium acetate
2	1	1	PEG 4000
3	1	2	PEG 4000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	2-7 (%(w/v))
2	1	reservoir	sodium acetate	0.075-0.100 (M)