1F46

THE BACTERIAL CELL-DIVISION PROTEIN ZIPA AND ITS INTERACTION WITH AN FTSZ FRAGMENT REVEALED BY X-RAY CRYSTALLOGRAPHY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-01-12
Detector	ADSC QUANTUM 4
Spacegroup name	P 1 21 1
Unit cell lengths	49.890, 41.740, 71.160
Unit cell angles	90.00, 98.26, 90.00

Refinement procedure

Resolution	20.000 - 1.500
R-factor	0.198
Rwork	0.198
R-free	0.21700
RMSD bond length	0.009
RMSD bond angle	1.340
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.063	0.199 *
Total number of observations	93040 *
Number of reflections	46702
<I/σ(I)>	19.8
Completeness [%]	99.5	97.8
Redundancy	3.81	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	18 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	reservoir	PEG6000	25 (%)
3	1	reservoir	MES	100 (mM)