1F3Z
IIAGLC-ZN COMPLEX
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-01 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 47.700, 47.700, 144.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.980 |
| Rwork | 0.193 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f3g |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.980 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | UNPUBLISHED |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.980 |
| Rmerge | 0.044 |
| Total number of observations | 22910 * |
| Number of reflections | 7578 |
| Completeness [%] | 61.0 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8.5 | 0.1-0.3 M SODIUM ACETATE, 1MM ZINC ACETATE, 0.1 M TRIS BUFFER PH 8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG4000 | 22-26 (%) | |
| 2 | 1 | 1 | sodium acetate | 0.1-0.3 (M) | |
| 3 | 1 | 1 | zinc acetate | 1 (mM) | |
| 4 | 1 | 1 | Tris-HCl | 0.1 (M) | |
| 5 | 1 | 2 | protain | 6 (mg/ml) |
