1F3W
RECOMBINANT RABBIT MUSCLE PYRUVATE KINASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08-15 |
| Detector | MAC Science DIP-2000H |
| Spacegroup name | P 1 |
| Unit cell lengths | 79.000, 105.400, 143.500 |
| Unit cell angles | 95.90, 92.80, 111.90 |
Refinement procedure
| Resolution | 52.000 - 3.000 |
| R-factor | 0.239 * |
| Rwork | 0.238 |
| R-free | 0.24700 * |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.082 | 0.258 |
| Number of reflections | 82732 | |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 98.4 | 82.9 * |
| Redundancy | 2.4 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 18 * | PEG 8000, manganese chloride, potassium chloride, sodium pyruvate, succinate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium pyruvate | 2.9 (mM) | |
| 2 | 1 | drop | 1.2 (mM) | ||
| 3 | 1 | drop | 0.45 (M) | ||
| 4 | 1 | drop | succinate adjusted | 30 (mM) | |
| 5 | 1 | drop | PEG8000 | 9-11 (%(w/v)) | |
| 6 | 1 | drop | protein | 1.5 (mg/ml) |
