1F3P

FERREDOXIN REDUCTASE (BPHA4)-NADH COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	100
Detector technology	DIFFRACTOMETER
Collection date	1998-12-11
Detector	WEISSENBERG
Spacegroup name	P 61 2 2
Unit cell lengths	98.900, 98.900, 170.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.000 - 2.400
R-factor	0.206 *
Rwork	0.205
R-free	0.29100
RMSD bond length	0.009
RMSD bond angle	1.400 *
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.085	0.478
Total number of observations	133802 *
Number of reflections	19786 *
<I/σ(I)>	8.9
Completeness [%]	99.6	99.2
Redundancy	6.7	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4 *	20 *	Yamada, T., (2000) Protein Pept.Lett., 7, 277. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	14.0 (mg/ml)
2	1	reservoir	sodium formate	2.0 (M)
3	1	reservoir	sodium acetate	100 (mM)