1F35
CRYSTAL STRUCTURE OF MURINE OLFACTORY MARKER PROTEIN
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2000-04-20 |
| Detector | BRANDEIS |
| Wavelength(s) | 0.9786, 0.9788, 0.9390, 1.078 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 87.270, 87.270, 164.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 2.300 |
| R-factor | 0.21 * |
| Rwork | 0.210 |
| R-free | 0.24700 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.400 |
| High resolution limit [Å] | 2.200 | 2.300 |
| Rmerge | 0.058 | 0.250 |
| Number of reflections | 35024 | |
| <I/σ(I)> | 2.69 | |
| Completeness [%] | 74.0 | 71.8 |
| Redundancy | 16.6 | 16.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | PEG 8000, Zinc Acetate, Cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 21K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | zinc acetate | 200 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 10 (%(w/v)) | |
| 4 | 1 | reservoir | cacodylate | 100 (mM) |
