1F2Y
SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 278 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-12-05 |
| Detector | MACSCIENCE |
| Spacegroup name | P 41 |
| Unit cell lengths | 48.400, 48.400, 63.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.690 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 6.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.072 |
| Number of reflections | 8212 |
| Completeness [%] | 86.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 8.15 | 298 | 10.5 MM POTASSIUM PHOSPHATE, MPD, pH 8.15, SMALL TUBES, temperature 298K |
