1F2T
Crystal Structure of ATP-Free RAD50 ABC-ATPase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-11-07 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.570, 67.080, 70.060 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.600 |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.23100 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | MLPHARE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.640 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.055 | 0.366 |
Number of reflections | 46541 * | |
<I/σ(I)> | 12.6 | |
Completeness [%] | 98.6 | 98.6 |
Redundancy | 4 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 * | 295 | 200 mM ammonium acetate, 50 mM cacodylate, 10 mM MgCl2, 10% PEG 4000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | drop | Na-phosphate | 20 (mM) | |
3 | 1 | drop | 300 (mM) | ||
4 | 1 | drop | EDTA | 1 (mM) | |
5 | 1 | reservoir | ammonium acetate | 200 (mM) | |
6 | 1 | reservoir | cacodylate | 50 (mM) | |
7 | 1 | reservoir | 10 (mM) | ||
8 | 1 | reservoir | PEG4000 | 10 (%) |