1F2K
CRYSTAL STRUCTURE OF ACANTHAMOEBA CASTELLANII PROFILIN II, CUBIC CRYSTAL FORM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 140 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-02-21 |
| Detector | FUJI |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 120.524, 120.524, 120.524 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.232 |
| Rwork | 0.229 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2acg |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.870 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.073 | 0.195 |
| Number of reflections | 13681 | |
| <I/σ(I)> | 18.3 | 7.2 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | AMMONIUM SULFATE, SODIUM CITRATE, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
