1F2J
CRYSTAL STRUCTURE ANALYSIS OF ALDOLASE FROM T. BRUCEI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 150 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-01-07 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 209.660, 209.660, 209.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.29300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | TETRAMERIC RABBIT ALDOLASE (1ADO) |
| RMSD bond length | 0.034 |
| RMSD bond angle | 4.084 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.500 | 2.100 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.092 | 0.121 |
| Total number of observations | 479727 * | |
| Number of reflections | 29298 * | |
| <I/σ(I)> | 21.3 | |
| Completeness [%] | 99.9 | 72.1 |
| Redundancy | 6.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 298 | 100 mM sodium acetate pH 4.9, 1.9 M ammonium sulfate, 20 mM L-cysteine, pH 4.8, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | PMSF | 100 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 1.9 (M) | |
| 7 | 1 | reservoir | L-cysteine | 20 (mM) | |
| 8 | 1 | reservoir | sodium acetate | 100 (mM) |
