1F1R
CRYSTAL STRUCTURE OF HOMOPROTOCATECHUATE 2,3-DIOXYGENASE FROM ARTHROBACTER GLOBIFORMIS (NATIVE, NON-CRYO)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1998-05-01 |
Detector | SIEMENS HI-STAR |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 138.440, 59.150, 102.800 |
Unit cell angles | 90.00, 118.26, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.156 |
Rwork | 0.156 |
R-free | 0.18700 |
RMSD bond length | 0.020 |
RMSD bond angle | 2.000 * |
Data reduction software | X-GEN |
Data scaling software | X-GEN |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.920 |
High resolution limit [Å] | 1.750 * | 1.850 |
Rmerge | 0.054 | 0.239 * |
Number of reflections | 60397 | |
<I/σ(I)> | 12.2 | |
Completeness [%] | 88.7 * | 65 * |
Redundancy | 2.6 * | 1.4 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 6.5 * | 18 * | Peg 8000, Mg Acetate, Na cacodylate, pH 6.8, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 100 (mg/ml) | |
2 | 1 | 2 | PEG8000 | 6-10 (%) | |
3 | 1 | 2 | magnesium acetate | 0.2 (M) | |
4 | 1 | 2 | sodium cacodylate | 100 (mM) | pH6.5 |