1F1F

CRYSTAL STRUCTURE OF CYTOCHROME C6 FROM ARTHROSPIRA MAXIMA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1995-06-01
Detector	RIGAKU RAXIS
Spacegroup name	P 21 21 21
Unit cell lengths	79.400, 67.800, 49.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.700
R-factor	0.232 *
Rwork	0.232
R-free	0.25500
RMSD bond length	0.011
RMSD bond angle	1.217
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.700	2.750
Rmerge	0.086
Number of reflections	7504
<I/σ(I)>	9.2
Completeness [%]	80.8	74
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	298	0.1 M Tris, pH 7.8, 2.4 M (NH4)2SO4, 0.5 M LiSO4, 1% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	Tris	0.1 (M)
3	1	reservoir	ammonium sulfate	2.4 (M)
4	1	reservoir	lithium sulfate	0.5 (M)
5	1	reservoir	glycerol	1 (%)