1F1E
CRYSTAL STRUCTURE OF THE HISTONE FROM METHANOPYRUS KANDLERI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-12 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.400, 57.400, 96.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.370 |
| R-factor | 0.163 * |
| Rwork | 0.159 |
| R-free | 0.20800 * |
| RMSD bond length | 0.016 * |
| RMSD bond angle | 2.880 * |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.390 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.064 | 0.475 |
| Number of reflections | 31694 | |
| <I/σ(I)> | 20.6 | |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 4.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 289 | Protein: 18mg/ml HMK, 1M NaCl, 50mM Tris pH 8.5, 50mM BME, Well: 400mM NaCl, 50mM Tris pH 8.5, 50mM BME, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | 1 (M) | ||
| 3 | 1 | drop | Tris | 50 (mM) | |
| 4 | 1 | drop | beta-mercaptoethanol | 1 (mM) | |
| 5 | 1 | reservoir | Tris | 50 (mM) | |
| 6 | 1 | reservoir | 400 (mM) |
