1F11
F124 FAB FRAGMENT FROM A MONOCLONAL ANTI-PRES2 ANTIBODY
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1999-04-01 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 135.700, 54.000, 120.000 |
Unit cell angles | 90.00, 101.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.000 |
R-factor | 0.184 * |
Rwork | 0.184 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1igi |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.131 | 0.479 |
Number of reflections | 17261 | 1665 * |
<I/σ(I)> | 6.7 | 1.8 |
Completeness [%] | 99.2 | 97.5 |
Redundancy | 1.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 4.6 | 290 | PEG 400, sodium acetate, ammonium sulfate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 2 (M) | |
2 | 1 | reservoir | sodium acetate | 0.1 (M) | |
3 | 1 | reservoir | PEG400 | 5 (%) | |
4 | 1 | drop | protein | 2.4 (mg/ml) | |
5 | 1 | drop | ammonium sulfate | 0.57 (M) | |
6 | 1 | drop | sodium acetate | 0.028 (M) | |
7 | 1 | drop | PEG400 | 1.4 (%) |