1F10
CRYSTAL STRUCTURE OF ORTHORHOMBIC LYSOZYME GROWN AT PH 6.5 AT 88% RELATIVE HUMIDITY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.584, 55.859, 68.583 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.700 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.26800 |
| Starting model (for MR) | ORTHORHOMBIC LYSOZYME AT PH 6.5 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 24.600 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.058 | 0.332 |
| Total number of observations | 54245 * | |
| Number of reflections | 13459 | 2083 * |
| <I/σ(I)> | 25 | 3.1 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40 (mg/ml) | |
| 2 | 1 | drop | phosphate | ||
| 3 | 1 | drop | 5 (%(w/v)) | ||
| 4 | 1 | reservoir | phosphate | ||
| 5 | 1 | reservoir | 20 (%(w/v)) |
