1F0W

CRYSTAL STRUCTURE OF ORTHORHOMBIC LYSOZYME GROWN AT PH 6.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Spacegroup name	P 21 21 21
Unit cell lengths	30.470, 59.390, 68.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.188
Rwork	0.188
R-free	0.27900
RMSD bond length	0.014
RMSD bond angle	1.900
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.400	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.064	0.475
Total number of observations	42921 *
Number of reflections	10122	1351 *
<I/σ(I)>	18	1.6
Completeness [%]	97.2	94.7
Redundancy	4.2	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5		pH 6.5
1	Vapor diffusion, hanging drop *	6.5		pH 6.5
1	Vapor diffusion, hanging drop *	6.5		pH 6.5
1	Vapor diffusion, hanging drop *	6.5		pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	drop	phosphate
3	1	drop		5 (%(w/v))
4	1	reservoir	phosphate
5	1	reservoir		20 (%(w/v))