1F0V
Crystal structure of an Rnase A dimer displaying a new type of 3D domain swapping
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.569, 96.341, 48.240 |
| Unit cell angles | 90.00, 107.50, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.700 |
| Rwork | 0.184 |
| R-free | 0.21300 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.072 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 * | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.052 | 0.080 |
| Total number of observations | 813673 * | |
| Number of reflections | 76047 | |
| <I/σ(I)> | 14 | |
| Completeness [%] | 95.0 | 95 |
| Redundancy | 10 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | drop contains protein and reservoir solution in a 1:1 ratio * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Phosphate buffer | ||
| 2 | 1 | 1 | PEG 4000 | ||
| 3 | 1 | 1 | Dioxane | ||
| 4 | 1 | 2 | Phosphate buffer | ||
| 5 | 1 | 2 | PEG 4000 | ||
| 6 | 1 | 2 | Dioxane |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | dCpdG | 2 (mM) | |
| 3 | 1 | drop | phosphate | 0.1 (M) | |
| 4 | 1 | reservoir | phosphate | 0.1 (M) | |
| 5 | 1 | reservoir | PEG4000 | 16 (%) | |
| 6 | 1 | reservoir | dioxane | 2 (%) |
