1F0O
PVUII ENDONUCLEASE/COGNATE DNA COMPLEX (GLUTARALDEHYDE-CROSSLINKED CRYSTAL) AT PH 7.5 WITH TWO CALCIUM IONS AT EACH ACTIVE SITE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-02-14 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 94.043, 84.702, 45.993 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.205 * |
| Rwork | 0.205 |
| R-free | 0.27900 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.057 | 0.332 |
| Total number of observations | 61937 * | |
| Number of reflections | 13150 | |
| <I/σ(I)> | 20.1 | |
| Completeness [%] | 98.9 | 96.5 |
| Redundancy | 4.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 16 * | Balendiran, K., (1994) Proteins Struct.Funct.Genet., 19, 77. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium acetate | ||
| 2 | 1 | 1 | CaCl2 | ||
| 3 | 1 | 1 | PEG 4000 | ||
| 4 | 1 | 2 | PEG 4000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | R-PvuII | 9.6 (mg/ml) | |
| 2 | 1 | drop | duplex DNA | 1.6 (mg/ml) | |
| 3 | 1 | drop | mother liquor | 18-25 (%) | |
| 4 | 1 | drop | PEG4000 | 8 (%) | mother liquor |
| 5 | 1 | drop | EDTA | 0.3 (mM) | mother liquor |
| 6 | 1 | drop | sodium acetate | 50 (mM) | |
| 7 | 1 | reservoir | mother liquor | 31-45 (%) |
