1F0N
MYCOBACTERIUM TUBERCULOSIS ANTIGEN 85B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-01-14 |
| Detector | ADSC |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 73.210, 73.210, 92.544 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.1941 |
| Rwork | 0.194 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ANTIGEN 85C RONNING ET AL. (2000) NAT.STRUCT.BIOL. 7(2) 141-146. |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.034 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.112 | 0.186 |
| Number of reflections | 26822 | |
| <I/σ(I)> | 12.7 | 6 |
| Completeness [%] | 97.6 | 97.4 |
| Redundancy | 4.8 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | Ammonium sulfate, MES, MPD, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 21K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 0.6-0.7 (M) | |
| 2 | 1 | reservoir | MPD | 22.5 (%) | |
| 3 | 1 | reservoir | Na-MES | 0.05 (M) |
