1F0K
THE 1.9 ANGSTROM CRYSTAL STRUCTURE OF E. COLI MURG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-11 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.613, 66.356, 67.902 |
| Unit cell angles | 64.29, 83.52, 65.45 |
Refinement procedure
| Resolution | 40.000 - 1.900 |
| Rwork | 0.220 |
| R-free | 0.24700 * |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.295 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.032 | 0.187 |
| Total number of observations | 288150 * | |
| Number of reflections | 65567 | |
| <I/σ(I)> | 41.9 | |
| Completeness [%] | 97.7 | 96.4 |
| Redundancy | 4.4 | 3.79 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 * | 298 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | EDTA | 50 (mM) | |
| 5 | 1 | reservoir | NaMES | 0.1 (M) | |
| 6 | 1 | reservoir | ammonium sulfate | 0.96 (M) | |
| 7 | 1 | reservoir | Triton-X-100 | 0.4 (%) | |
| 8 | 1 | reservoir | dithiothreitol | 10 (mM) |
