1EZL
CRYSTAL STRUCTURE OF THE DISULPHIDE BOND-DEFICIENT AZURIN MUTANT C3A/C26A: HOW IMPORTANT IS THE S-S BOND FOR FOLDING AND STABILITY?
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 295 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1998-01-01 |
| Detector | SIEMENS P4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.400, 80.400, 110.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| Rwork | 0.189 |
| R-free | 0.25600 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.970 |
| Data reduction software | SMART |
| Data scaling software | SAINT |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 8.000 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.083 | 0.083 |
| Total number of observations | 255491 * | |
| Number of reflections | 39662 | |
| Completeness [%] | 93.0 | 93 |
| Redundancy | 6.4 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 295 | ammonium sulphate, lithium nitrate, acetate, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 3.4 (M) | |
| 2 | 1 | reservoir | lithium nitrate | 0.5 (M) | |
| 3 | 1 | reservoir | acetate | 0.1 (M) |
