1EZ9

STRUCTURE OF MALTOTETRAITOL BOUND TO OPEN-FORM MALTODEXTRIN BINDING PROTEIN IN P1 CRYSTAL FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-12-01
Detector	MACSCIENCE
Spacegroup name	P 1
Unit cell lengths	51.745, 57.900, 64.594
Unit cell angles	89.31, 81.78, 71.97

Refinement procedure

Resolution	15.000 - 1.900
R-factor	0.192 *
Rwork	0.192
R-free	0.23500
RMSD bond length	0.010 *
RMSD bond angle	1.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.041 *
Number of reflections	51060
Completeness [%]	91.9 *	79.8
Redundancy	1.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	298	PEG 3350, MES, sodium azide, maltotetraitol, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MBP	3.5 (mg/ml)
2	1	drop	maltotetraitol	1 (mM)
3	1	drop	PEG3350	20 (%)	pH6.6
4	1	reservoir	PEG3350	30 (%)