1EZ1
STRUCTURE OF ESCHERICHIA COLI PURT-ENCODED GLYCINAMIDE RIBONUCLEOTIDE TRANSFORMYLASE COMPLEXED WITH MG, AMPPNP, AND GAR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 120 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 2000-02-11 |
| Detector | SIEMENS P4 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 61.800, 179.400, 75.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.750 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.25400 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 17.100 * |
| Data scaling software | SAINT |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.040 | 0.140 |
| Number of reflections | 83477 | |
| <I/σ(I)> | 20.6 | |
| Completeness [%] | 96.6 | 93.5 |
| Redundancy | 3.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 4 * | methylether PEG-5000, 5'-adenylylimidodiphosphate, 3-(N-morpholino)propanesulfonic acid, sodium chloride, magnesium chloride, glycinamide ribonucleotide, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | AMPPNP | 5 (mM) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | 250 (mM) | ||
| 5 | 1 | reservoir | mPEG5000 | 18-22 (%(w/v)) | |
| 6 | 1 | reservoir | MOPS | 100 (mM) | |
| 7 | 1 | reservoir | 100 (mM) |
