1EYZ
STRUCTURE OF ESCHERICHIA COLI PURT-ENCODED GLYCINAMIDE RIBONUCLEOTIDE TRANSFORMYLASE COMPLEXED WITH MG AND AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 120 |
| Detector technology | AREA DETECTOR |
| Collection date | 2000-01-25 |
| Detector | SIEMENS HI-STAR |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 62.300, 179.500, 75.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.750 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.22800 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 17.500 * |
| Data scaling software | SAINT |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.067 | 0.269 |
| Number of reflections | 83063 * | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 96.1 | 90.8 |
| Redundancy | 3.2 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 4 * | methylether PEG-5000, sodium chloride, magnesium chloride, 5'-adenylylimidodiphosphate, 3-(N-morpholino)propanesulfonic acid, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | AMPPNP | 5 (mM) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | 250 (mM) | ||
| 5 | 1 | reservoir | mPEG5000 | 18-22 (%(w/v)) | |
| 6 | 1 | reservoir | MOPS | 100 (mM) | |
| 7 | 1 | reservoir | 100 (mM) |
