1EYP
CHALCONE ISOMERASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-01-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 90.370, 90.370, 352.860 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.100 - 2.500 |
| R-factor | 0.235 * |
| Rwork | 0.249 |
| R-free | 0.28000 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.100 | 2.610 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.063 | 0.407 |
| Total number of observations | 86781 * | |
| Number of reflections | 27821 | |
| <I/σ(I)> | 19.4 | |
| Completeness [%] | 90.7 | 61.7 * |
| Redundancy | 3.1 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 4 * | 25% glycerol 1.8 M ammonium sulfate 0.05 M PIPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 302K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | reservoir | glycerol | 25 (%(v/v)) | |
| 3 | 1 | reservoir | ammonium sulfate | 1.8-2.0 (M) | |
| 4 | 1 | reservoir | PIPES | 0.05 (M) | pH6.5 |
