1EYJ
FRUCTOSE-1,6-BISPHOSPHATASE COMPLEX WITH AMP, MAGNESIUM, FRUCTOSE-6-PHOSPHATE AND PHOSPHATE (T-STATE)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | SIEMENS |
Temperature [K] | 120 |
Detector technology | AREA DETECTOR |
Collection date | 1998-09-01 |
Detector | SIEMENS |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 59.860, 165.820, 79.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 5.000 - 2.280 |
Rwork | 0.197 |
R-free | 0.25500 |
RMSD bond length | 0.006 |
RMSD bond angle | 23.000 * |
Data reduction software | X-GEN |
Data scaling software | X-GEN |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.360 |
High resolution limit [Å] | 2.280 | 2.230 |
Rmerge | 0.106 | 0.198 |
Total number of observations | 122394 * | |
Number of reflections | 36815 | |
<I/σ(I)> | 7.1 | |
Completeness [%] | 99.0 | 94 |
Redundancy | 3.4 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 310 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | 10 (mM) | ||
3 | 1 | drop | 2 (mM) | or 5 mM MgCl2 | |
4 | 1 | drop | F6P | 5 (mM) | |
5 | 1 | drop | AMP | 5 (mM) | |
6 | 1 | reservoir | HEPES | 100 (mM) | |
7 | 1 | reservoir | PEG3350 | 10 (%(w/v)) |