1EYB
CRYSTAL STRUCTURE OF APO HUMAN HOMOGENTISATE DIOXYGENASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-03-18 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 158.380, 158.380, 93.459 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.22200 |
| RMSD bond length | 0.001 |
| RMSD bond angle | 1.650 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.061 | 0.292 |
| Total number of observations | 503991 * | |
| Number of reflections | 96251 * | |
| <I/σ(I)> | 18.4 | |
| Completeness [%] | 93.0 | 94.5 |
| Redundancy | 9.9 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 298 | Ammonium sulfate, imidazole, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | ammonium sulfate | 1.5-2.0 (M) |
