1EXZ

STRUCTURE OF STEM CELL FACTOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	1999-11-26
Detector	BRANDEIS
Spacegroup name	P 1 21 1
Unit cell lengths	36.154, 87.526, 79.434
Unit cell angles	90.00, 97.76, 90.00

Refinement procedure

Resolution	40.000 - 2.300
R-factor	0.223
Rwork	0.223
R-free	0.29400
RMSD bond length	0.007
RMSD bond angle	1.220
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.058	0.250
Number of reflections	21454
<I/σ(I)>	29
Completeness [%]	99.5	90.1
Redundancy	8	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	20 *	drop consists of equal volume of protein and reservoir solutions *
1	VAPOR DIFFUSION, HANGING DROP	7	20 *	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15-20 (mg/ml)
2	1	reservoir	PEG400	25-30 (%)
3	1	reservoir		0.25 (M)
4	1	reservoir	HEPES	0.1 (M)
5	1	drop		1 (mM)