1EXU

CRYSTAL STRUCTURE OF THE HUMAN MHC-RELATED FC RECEPTOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1998-06-03
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 43 21 2
Unit cell lengths	66.387, 66.387, 221.813
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.700
Rwork	0.197
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1FRU
RMSD bond length	0.008
RMSD bond angle	29.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.857)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.067	0.307
Total number of observations	79025 *
Number of reflections	14245	1374 *
<I/σ(I)>	23.5
Completeness [%]	98.6	99.4
Redundancy	5.6	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5 *	22 *	ammonium sulfate, glycerol, tris, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	21 (mg/ml)
2	1	reservoir	ammonium sulfate	1.5 (M)
3	1	reservoir	Tris-HCl	100 (mM)
4	1	reservoir	glycerol	12 (%(w/v))