1EXS

STRUCTURE OF PORCINE BETA-LACTOGLOBULIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-03-15
Detector	MARRESEARCH
Spacegroup name	P 32 2 1
Unit cell lengths	80.605, 80.605, 78.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 2.390
R-factor	0.218 *
Rwork	0.219
R-free	0.28200
RMSD bond length	0.013
RMSD bond angle	3.049
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000 *	2.440
High resolution limit [Å]	2.390 *	2.390 *
Rmerge	0.052	0.250 *
Total number of observations	124487 *
Number of reflections	12225	870 *
<I/σ(I)>	9.7
Completeness [%]	99.8	97.3
Redundancy	10.2	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	3.2	293	Sodium chloride, Formate buffer, pH 3.2, microbatch, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	12.5 (mg/ml)
2	1	1		1.3 (M)
3	1	1	formate	100 (mM)	pH3.2