1EXJ
CRYSTAL STRUCTURE OF TRANSCRIPTION ACTIVATOR BMRR, FROM B. SUBTILIS, BOUND TO 21 BASE PAIR BMR OPERATOR AND TPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-09-24 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 109.490, 109.490, 144.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.000 - 3.000 |
| Rwork | 0.240 |
| R-free | 0.31500 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.500 |
| Data reduction software | bioteX |
| Data scaling software | bioteX |
| Phasing software | PHASES |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.200 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.094 | 0.275 |
| Number of reflections | 55012 | |
| <I/σ(I)> | 5.5 | |
| Completeness [%] | 88.0 | 65 |
| Redundancy | 3.4 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 * | 293 | 1 M imidazole, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | imidazole | ||
| 2 | 1 | 2 | imidazole |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mM) | |
| 2 | 1 | drop | DMSO | 1 (%) | |
| 3 | 1 | reservoir | imidazole | 1.0-1.2 (M) |
